Systematics Section / ASPT
Kim, Sangtae , Zahn, Laura M. , Chanderbali, Andre , Zheng, Zhengui , Oppenheimer, David , Ma, Hong , Soltis, Douglas E. , Soltis, Pamela S. .
The evolution of the AGL6-like MADS-box genes.
AGL6-LIKE genes form one of the major subgroups of the MADS-box gene family and are closely related to the SEP (E-class) and SQUA (A-class) subfamilies of MADS-box genes. In Arabidopsis, AGL6 and AGL13 have been reported from the AGL6 subfamily, but detailed expression studies of these genes have not yet been performed. We identified several new AGL6-like genes from pivotal taxa in angiosperm phylogeny and analyzed them together with previously reported AGL6-like genes. Structural analyses showed 1) a one-aa gap in the I-domain in all AGL6-like genes relative to SEP-like and SQUA-like genes, 2) a seven-aa insertion in the C-domain of genes from asterids, and 3) a one-aa insertion in the C-domain of genes from gymnosperms. Phylogenetic analyses showed that AGL6-like genes are sister to AGL2-like genes, and SQUA-like genes are sister to these two groups. These relationships were strongly supported in both amino acid and DNA analyses. The phylogenetic tree of AGL6-like genes generally tracks organismal phylogeny as inferred from multigene data sets. Unlike other subgroups of MADS-box genes (e.g., DEF, SQUA, and SEP), no major duplication of AGL6-like genes occurred in the common ancestor of core eudicots. However, several recent gene duplications were detected (e.g., within Magnoliaceae). We investigated the expression of AGL6 genes from Arabidopsis, Eschscholzia, Magnolia, Liriodendron, Persea, Nuphar, and Amborella using relative-quantitative RT-PCR, in situ hybridization, and microarrays and consider the expression patterns in a phylogenetic context.
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1 - University of Florida, Department of Botany, 220 Bartram Hall, P.O. Box 118526, Gainesville, Florida, 32611-8526, USA
2 - Pennsylvania State University, Department of Biology, 403 Life Sciences Building, University Park, Pennsylvania, 16802, USA
3 - University of Florida, Department of Botany, Florida Museum of Natural History, P.O. Box 117800, Gainesville, Florida, 32611-7800, USA
4 - Pennsylvania State University, Department of Biology, 202 Mueller Lab, University Park, Pennsylvania, 16802-5301, USA
in situ hybridization
Presentation Type: Poster:Posters for Sections
Location: Auditorium/Bell Memorial Union
Date: Tuesday, August 1st, 2006
Time: 12:30 PM