Spielbauer, Kristen A. , Smith-Huerta, Nancy L. .
The use of NanoDrop technology to measure nectar concentration in flowers.
DETERMINING nectar concentration in flowers forms the foundation for many pollination biology studies. The most commonly used methods to determine nectar concentration include the anthrone method, or the use of hand-held refractometers. Although accurate, both methods have limitations. The anthrone method does not allow concentrated samples to be diluted for spectrophotometric analysis once they have been processed. Refractometers often require relatively large nectar quantities to obtain accurate measurements. In response to these problems, we developed a method to determine nectar concentration in flowers using the DNSA reagent and NanoDrop technology. Our method allows nectar samples less than 1 microliter in volume to be accurately measured. In addition, the DNSA reagent does not form a precipitate, allowing dilution of both standards and samples if too concentrated. Finally, the NanoDrop spectrophotometer requires only 2 microliters of solution to make a reading, far less than required by a standard spectrophotometer.
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1 - Miami University, Department of Botany, Oxford, Ohio, 45056, USA
Presentation Type: Poster:Posters for Sections
Location: Auditorium/Bell Memorial Union
Date: Tuesday, August 1st, 2006
Time: 12:30 PM